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G protein beta interacts with the glucocorticoid receptor and suppresses its transcriptional activity in the nucleus

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Title: G protein beta interacts with the glucocorticoid receptor and suppresses its transcriptional activity in the nucleus
Author(s): Kino, Tomoshige; Tiulpakov, Anatoly; Ichijo, Takamasa; Chheng, Ly; Kozasa, Tohru; Chrousos, George P.
Subject(s): pituitary-adrenal axis steroid-hormones somatostatin rack1
Abstract: Extracellular stimuli that activate cell surface receptors modulate glucocorticoid actions via as yet unclear mechanisms. Here, we report that the guanine nucleotide-binding protein (G protein)-coupled receptor-activated WD-repeat G beta interacts with the glucocorticoid receptor (GR), comigrates with it into the nucleus and suppresses GR-induced transactivation of the glucocorticoid-responsive genes. Association of G gamma with G beta is necessary for this action of G beta. Both endogenous and enhanced green fluorescent protein (EGFP)-fused G beta 2 and G gamma 2 proteins were detected in the nucleus at baseline, whereas E a fraction of EGFP-G beta 2 and DsRed2-GR comigrated to the nucleus or the plasma membrane, depending on the exposure of cells to dexamethasone or somatostatin, respectively. G beta 2 was associated with GR/glucocorticoid response elements (GREs) in vivo and suppressed activation function-2-directed transcriptional activity of the GR. We conclude that the G beta gamma complex interacts with the GR and suppresses its transcriptional activity by associating with the transcriptional complex formed on GR-responsive promoters.
Issue Date: 2005-06
Publisher: Rockefeller University Press
Citation Info: Kino, T., A. Tiulpakov, T. Ichijo, L. Chheng, T. Kozasa, and G. P. Chrousos, 2005, G protein beta interacts with the glucocorticoid receptor and suppresses its transcriptional activity in the nucleus: Journal of Cell Biology, v. 169, no. 6, p. 885-896. http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=pmcentrez&artid=2171637&blobtype=pdf
Type: Article
Description: http://www.jcb.org/misc/copyright.pdf
URI: http://hdl.handle.net/10027/1405
ISSN: 0021-9525
Date Available in INDIGO: 2008-07-14
 

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