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Rapid quantitative analysis of 8-iso-prostaglandin-F(2alpha) using liquid chromatography-tandem mass spectrometry and comparison with an enzyme immunoassay method

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Title: Rapid quantitative analysis of 8-iso-prostaglandin-F(2alpha) using liquid chromatography-tandem mass spectrometry and comparison with an enzyme immunoassay method
Author(s): Dahl, Jeffrey H.; van Breemen, Richard B.
Subject(s): mass spectrometry enzyme immunoassay quantitative analysis biomarker
Abstract: A rapid liquid chromatography-tandem mass spectrometry (LC-MS-MS) assay was developed for the measurement of urinary 8-iso prostaglandin F2α (8-iso-PGF2α), a biomarker of lipid peroxidation. Since urine contains numerous F2 prostaglandin isomers, each of identical mass and similar mass spectrometric fragmentation patterns, chromatographic separation of 8-iso-PGF2α from its isomers is necessary for its quantitative analysis using tandem mass spectrometry. We were able to achieve this separation using an isocratic LC method with a run time under nine minutes which is at least three-fold faster than previous methods—while maintaining sensitivity, accuracy, precision and reliability. The limits of detection and quantitation were 53 and 178 pg/mL urine, respectively. We compared our method with a commercially available affinity purification and enzyme immunoassay kit and found both assays in agreement. Despite the high sensitivity of the enzyme immunoassay method, it is more expensive and has a narrower dynamic range than LC-MS-MS. Our method was optimized for rapid measurement of 8-iso-PGF2α in urine, and it is ideally suited for clinical sample analysis.
Issue Date: 2010-09-15
Publisher: Elsevier
Citation Info: Dahl, J. H. & Breemen, R. B. 2010. Rapid quantitative analysis of 8-iso-prostaglandin-F(2alpha) using liquid chromatography-tandem mass spectrometry and comparison with an enzyme immunoassay method. Analytical Biochemistry. 404(2):211-6. DOI: 10.1016/j.ab.2010.05.023
Type: Article
Description: Post print version of article may differ from published version. The definitive version is available through Elsevier at DOI: 10.1016/j.ab.2010.05.023
URI: http://hdl.handle.net/10027/7359
ISSN: 1096-0309
Sponsor: Funding for this research was provided by grant number 5R01CA101052 from the National Cancer Institute.
Date Available in INDIGO: 2011-03-01
 

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