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The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cells

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Title: The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cells
Author(s): Rapier, Rebecca; Huq, Jameela; Vishnubhotla, Ramana; Bulic, Marinka; Perrault, Cecile M.; Metlushko, Vitali; Cho, Michael; Tay, Roger T. S.; Glover, Sarah C.
Subject(s): microtopography actin cytoskeletal turnover
Abstract: We have shown that the microtopography (mT) underlying colon cancer changes as a tumor de-differentiates. We distinguish the well-differentiated mT based on the increasing number of "pits" and poorly differentiated mT on the basis of increasing number of "posts." We investigated Rho A as a mechanosensing protein using mT features derived from those observed in the ECM of colon cancer. We evaluated Rho A activity in less-tumorogenic (Caco-2 E) and more tumorigenic (SW620) colon cancer cell-lines on microfabricated pits and posts at 2.5 mu m diameter and 200 nm depth/height. In Caco-2 E cells, we observed a decrease in Rho A activity as well as in the ratio of G/F actin on surfaces with either pits or posts but despite this low activity, knockdown of Rho A led to a significant decrease in confined motility suggesting that while Rho A activity is reduced on these surfaces it still plays an important role in controlling cellular response to barriers. In SW620 cells, we observed that Rho A activity was greatest in cells plated on a post microtopography which led to increased cell motility, and an increase in actin cytoskeletal turnover.
Issue Date: 2010-07-28
Publisher: BioMed Central
Citation Info: Rapier, R., Huq, J., Vishnubhotla, R., Bulic, M., Perrault, C. M., Metlushko, V., Cho, M., Tay, R. T. S., & Glover, S. C. 2010. The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cells. Cancer Cell International, 10. DOI: 10.1186/1475-2867-10-24
Type: Article
Description: Post print version of article may differ from published version. The definitive version is available through BioMed Central at DOI: 10.1186/1475-2867-10-24
URI: http://hdl.handle.net/10027/7564
ISSN: 1475-2867
Sponsor: We are grateful to the American Gastroenterological Association, the University of Illinois GILD, and the National Institutes of Health (1 RO1 CA113975-A2) for funding this work.
Date Available in INDIGO: 2011-04-29
 

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