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Magnetization Transfer Imaging Provides a Quantitative Measure of Chondrogenic Differentiation and Tissue Development

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Title: Magnetization Transfer Imaging Provides a Quantitative Measure of Chondrogenic Differentiation and Tissue Development
Author(s): Li, Weiguo; Hong, Liu; Hu, Liping; Magin, Richard L.
Abstract: The goal of the present investigation was to test whether quantitative magnetization transfer imaging can be used as a noninvasive evaluation method for engineered cartilage. In this work, we used magnetic resonance imaging (MRI) to monitor the chondrogenesis of stem-cell-based engineered tissue over a 3-week period by measuring on a pixel-by-pixel basis the relaxation times (T-1 and T-2), the apparent diffusion coefficient, and the magnetization transfer parameters: bound proton fraction and cross-relaxation rate (k). Tissue-engineered constructs for generating cartilage were created by seeding mesenchymal stem cells in a gelatin sponge. Every 7 days, tissue samples were analyzed using MRI, histological, and biochemical methods. The MRI measurements were verified by histological analysis, and the imaging data were correlated with biochemical analysis of the developing cartilage matrix for glycosaminoglycan content. The MRI analysis for bound proton fraction and k showed a statistically significant increase that was correlated with the increase of glycosaminoglycan (R = 0.96 and 0.87, respectively, p < 0.05), whereas T-1, T-2, and apparent diffusion coefficient results did not show any significant changes over the 3-week measurement period.
Issue Date: 2010-12
Publisher: Mary Ann Liebert
Citation Info: Li, W. G., Hong, L., Hu, L. P., & Magin, R. L. 2010. Magnetization Transfer Imaging Provides a Quantitative Measure of Chondrogenic Differentiation and Tissue Development. Tissue Engineering Part C-Methods , 16(6): 1407-1415. DOI: 10.1089/ten.tec.2009.0777.
Type: Article
Description: This is a copy of an article published in the Tissue Engineering Part C Methods © 2010 [copyright Mary Ann Liebert, Inc.]; Tissue Engineering Part C Methods is available online at: http://www.liebertonline.com. DOI: 10.1089/ten.tec.2009.0777
URI: http://hdl.handle.net/10027/7624
ISSN: 1937-3384
Sponsor: This research was supported by the National Institute of Biomedical Imaging and Bioengineering under award number R01-EB007537 and a Grant-in-Aid for Mr. Weiguo Li from Sigma Xi.
Date Available in INDIGO: 2011-05-12
 

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