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Ultrashort pulse laser ablation for depth profiling of bacterial biofilms

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Title: Ultrashort pulse laser ablation for depth profiling of bacterial biofilms
Author(s): Milasinovic, Slobodan; Liu, Yaoming; Gasper, Gerald L.; Zhao, Youbo; Johnston, Joanna L.; Gordon, Robert J.; Hanley, Luke
Subject(s): bacteria biofilm
Abstract: Sample ablation by pulsed lasers is one option for removing material from a sample surface for in situ depth profiling during imaging mass spectrometry, but ablation is often limited by laser-induced damage of the remaining material. A preliminary evaluation was performed of sub-100 fs, 800 nm pulsed laser ablation for depth profiling of bacterial biofilms grown on glass by the drip flow method. Electron and optical microscopy were combined with laser desorption vacuum ultraviolet postionization mass spectrometry to analyze biofilms before and after ablation. Ultrashort laser pulses can ablate 10 – 100 μm thick sections of bacterial biofilms, leaving behind a layer of lysed cells. However, mass spectra from intact and ablated biofilms doped with antibiotic are almost identical, indicating little chemical degradation by ablation. These results are consistent with prior observations from laser surgery and support the use of ultrashort pulse laser ablation for minimally disruptive depth profiling of bacterial biofilms and intact biological samples.
Issue Date: 2010-07-01
Publisher: American Vacuum Society
Citation Info: Milasinovic, S., Liu, Y., Gasper, G. L., Zhao, Y., Johnston, J. L., Gordon, R. J., & Hanley, L. 2010. Ultrashort Pulse Laser Ablation for Depth Profiling of Bacterial Biofilms. Journal of Vacuum Science and Technology a Vacuum Surfaces and Films, 28(4): 647-651. DOI: 10.1116/1.3397736
Type: Article
Description: Post print version of article may differ from published version. The definitive version is available through American Vacuum Society at DOI: 10.1116/1.3397736
URI: http://hdl.handle.net/10027/7642
ISSN: 0734-2101
Sponsor: This work was supported by the National Institute of Biomedical Imaging and Bioengineering via grant EB006532.
Date Available in INDIGO: 2011-05-25
 

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