INDIGO Home University of Illinois at Urbana-Champaign logo uic building uic pavilion uic student center

Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Upregulate Myelin Basic Protein in Shiverer Mice

Show simple item record

Bookmark or cite this item: http://hdl.handle.net/10027/7727

Files in this item

File Description Format
PDF Human Umbilical ... otein in Shiverer Mice.pdf (657KB) (no description provided) PDF
Title: Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Upregulate Myelin Basic Protein in Shiverer Mice
Author(s): Veeravalli, Krishna Kumar; Dasari, Venkata Ramesh; Fassett, Daniel; Dinh, Dzung H.; Rao, Jasti S.
Subject(s): umbilical cord stem cell
Abstract: Human umbilical cord blood is a rich source of pluripotent mesenchymal stem cells and possesses significant advantages over other stem cell sources such as the embryo and bone marrow. In the present study, we aimed to investigate the potential of human umbilical cord blood-derived pluripotent mesenchymal stem cells (hUCB) to myelinate the axons of shiverer mice brains. We also investigated the effect of hUCB treatment on regulation of myelin basic protein in vitro in PC-12 cells, which are normally not myelinated. The results of our study clearly demonstrated that hUCB survive and migrate in vivo and has the potential to myelinate shiverer mice brains. The expression level of myelin basic protein, a major component of the myelin sheath, has been significantly increased in vivo and in vitro as revealed by Western blot, reverse transcription (RT)-polymerase chain reaction, immunohistochemistry, immunocytochemistry, and fluorescent in situ hybridization results. Further, transmission electron microscopic images of hUCB-treated shiverer mice brains showed several layers of myelin around the axons compared with a thin and fragmented layer of myelin in untreated animals. Moreover, the frequency of shivering was diminished 1 month after hUCB treatment in shiverer mice. Our results strongly indicated that hUCB transplantation could be an effective means of treating demyelinating or hypomyelinating disorders.
Issue Date: 2010-11-30
Publisher: Mary Ann Liebert
Citation Info: Veeravalli, K. K., Dasari, V. R., Fassett, D., Dinh, D. H., & Rao, J. 2010. Human umbilical cord blood derived mesenchymal stem cells upregulate myelin basic protein in shiverer mice. Stem Cells and Development. DOI: 10.1089/scd.2010.0187.
Type: Article
Description: This is a copy of an article published in the Stem Cells and Development © 2010 [copyright Mary Ann Liebert, Inc.]; Stem Cells and Development is available online at: http://www.liebertonline.com. DOI: 10.1089/scd.2010.0187.
URI: http://hdl.handle.net/10027/7727
ISSN: 1547-3287
Sponsor: This research was supported by a grant from the Illinois Regenerative Medicine Institute, a program of the Illinois Department of Public Health funded through the Illinois Department of Healthcare and Family Services.
Date Available in INDIGO: 2011-05-27
 

This item appears in the following Collection(s)

Show simple item record

Statistics

Country Code Views
United States of America 155
China 52
United Kingdom 14
Netherlands 6
Russian Federation 4

Browse

My Account

Information

Access Key