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Crystallization and preliminary X-ray characterization of the glpX-encoded class II fructose-1,6-bisphosphatase from Mycobacterium tuberculosis

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Title: Crystallization and preliminary X-ray characterization of the glpX-encoded class II fructose-1,6-bisphosphatase from Mycobacterium tuberculosis
Author(s): Gutka, Hiten J.; Franzblau, Scott G.; Movahedzadeh, Farahnaz; Abad-Zapatero, Cele
Subject(s): crystallization Mycobacterium tuberculosis X-ray characterization
Abstract: Fructose-1,6-bisphosphatase (FBPase; EC 3.1.3.11), which is a key enzyme in gluconeogenesis, catalyzes the hydrolysis of fructose 1,6-bisphosphate to form fructose 6-phosphate and orthophosphate. The present investigation reports the crystallization and preliminary crystallographic studies of the glpX-encoded class II FBPase from Mycobacterium tuberculosis H37Rv. The recombinant protein, which was cloned using an Escherichia coli expression system, was purified and crystallized using the hanging-drop vapor-diffusion method. The crystals diffracted to a resolution of 2.7 Å and belonged to the hexagonal space group P6122, with unit-cell parameters a = b = 131.3, c = 143.2 Å. The structure has been solved by molecular replacement and is currently undergoing refinement.
Issue Date: 2011-06
Publisher: International Union of Crystallography
Citation Info: Gutka, H. J., Franzblau, S. G., Movahedzadeh, F., & Abad-Zapatero, C. 2011. Crystallization and preliminary X-ray characterization of the glpX-encoded class II fructose-1,6-bisphosphatase from Mycobacterium tuberculosis. Acta Crystallographica Section F-Structural Biology and Crystallization Communications, 67: 710-713. DOI: 10.1107/S1744309111014722
Type: Article
Description: The original version is available through International Union of Crystallography at DOI: 10.1107/S1744309111014722.
URI: http://hdl.handle.net/10027/8192
ISSN: 1744-3091
Sponsor: The authors are grateful to the American Lung Association (Grant No. RG-82534-N) for their support. Supporting institutions may be found at http://www.ser-cat.org/members.html. Use of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. W-31-109-Eng-38.
Date Available in INDIGO: 2012-03-09
 

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