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Efficient Induction of CD25(-) iTreg by Co-Immunization Requires Strongly Antigenic Epitopes for T Cells

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Title: Efficient Induction of CD25(-) iTreg by Co-Immunization Requires Strongly Antigenic Epitopes for T Cells
Author(s): Geng, Shuang; Yu, Yang; Kang, Youmin; Pavlakis, George; Jin, Huali; Li, Jinyao; Hu, Yanxin; Hu, Weibin; Wang, Shuang; Wang, Bin
Subject(s): CD25(-) iTreg antigenic epitopes
Abstract: Background: We previously showed that co-immunization with a protein antigen and a DNA vaccine coding for the same antigen induces CD40(low) IL-10(high) tolerogenic DCs, which in turn stimulates the expansion of antigenspecific CD4(+)CD25(-)Foxp3(+) regulatory T cells (CD25(-) iTreg). However, it was unclear how to choose the antigen sequence to maximize tolerogenic antigen presentation and, consequently, CD25(-) iTreg induction. Results: In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25(-) iTreg induction. Firstly, we showed that the induction of CD25(-) iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25(-) iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25(-) iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes. Conclusions: Our data thus indicate that efficient induction of CD25- iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25- iTreg for clinical applications such as flea allergic dermatitis.
Issue Date: 2011-05-05
Publisher: BioMed Central
Citation Info: Geng, S. A., Yu, Y., Kang, Y. M., Pavlakis, G., Jin, H. L., Li, J. Y., Hu, Y. X., Hu, W. B., Wang, S. A., & Wang, B. 2011. Efficient induction of CD25(-) iTreg by co-immunization requires strongly antigenic epitopes for T cells. BMC Immunology, 12(27). DOI: 10.1186/1471-2172-12-27
Type: Article
Description: © 2011 Geng et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/license/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The original version is available through BioMed Central at DOI: 10.1186/1471-2172-12-27.
URI: http://hdl.handle.net/10027/8231
ISSN: 1471-2172
Sponsor: This work was supported in part by the National Nature Science Foundation of China (20771602 and 30930068) and State Key Lab Innovative Research initiation (2008SKLAB05-02) to BW.
Date Available in INDIGO: 2012-03-21
 

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