INDIGO Home University of Illinois at Urbana-Champaign logo uic building uic pavilion uic student center

Regulation of plasma cholesterol esterification by sphingomyelin: Effect of physiological variations of plasma sphingomyelin on lecithin-cholesterol acyltransferase activity.

Show full item record

Bookmark or cite this item: http://hdl.handle.net/10027/8535

Files in this item

File Description Format
PDF nps7AFB.pdf (18KB) figure 1 PDF
PDF nps84B3.pdf (22KB) figure 3 PDF
PDF nps3FD8.pdf (16KB) figure 4 PDF
PDF nps11F3.pdf (25KB) figure 2 PDF
PDF npsF400.pdf (80KB) main article PDF
PDF nps4AD2.pdf (151KB) figure 5 PDF
PDF npsD112.pdf (43KB) figure 6 PDF
Title: Regulation of plasma cholesterol esterification by sphingomyelin: Effect of physiological variations of plasma sphingomyelin on lecithin-cholesterol acyltransferase activity.
Author(s): Subbaiah, Papasani Venkata; Jiang, Xian-Cheng; Belikova, Natalia A.; Aizezi, Buzulagu; Huang, Zhi Hua; Reardon, Catherine A.
Abstract: Although sphingomyelin (SM) is the most abundant phospholipid in the plasma, next to phosphatidylcholine (PC), its physiological function in plasma is unclear. Here we employed plasma from various genetic models of mice which naturally differ in their plasma SM/PC ratios, to study the role of SM as a modulator of LCAT, the enzyme responsible for HDL maturation and the synthesis of cholesteryl esters (CE) in normal plasma. Serine palmitoyltransferase deficient mice, and SM synthase deficient mice, both of which have below normal SM/PC ratios, showed significantly elevated LCAT activities when assayed with the endogenous substrates. On the other hand, LDL receptor knockout mice, and apo E knockout mice, both of which have high SM/PC ratios, had markedly reduced (-80%) LCAT activities. The LCAT levels in plasma, as assayed with an exogenous substrate, were similar in all groups, except for a 45% decrease in apo E knockout mice. Plasma samples with high SM/PC ratios had lower percentage of 20:4, 22:5, and 22:6 CE all of which are formed by LCAT, and a higher percentage of the atherogenic 18:1 CE which is mainly derived from the action of liver ACAT, showing that in vivo, the contribution of LCAT to plasma CE is reduced while that of liver ACAT is increased. These results show that SM is a physiological modulator of LCAT activity as well as plasma CE composition, and this may contribute to the previously reported pro-atherogenic effect of high plasma SM levels.
Issue Date: 2012-06
Publisher: Elsevier
Citation Info: Subbaiah, P. V., Jiang, X. C., Belikova, N. A., Aizezi, B., Huang, Z. H., & Reardon, C. A. 2012. Regulation of plasma cholesterol esterification by sphingomyelin: Effect of physiological variations of plasma sphingomyelin on lecithin-cholesterol acyltransferase activity. BBA - Biochimica et Biophysica Acta, 1821(6): 908-913. DOI: 10.1016/j.bbalip.2012.02.007
Type: Article
Description: NOTICE: this is the author’s version of a work that was accepted for publication in BBA - Biochimica et Biophysica Acta . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in BBA - Biochimica et Biophysica Acta , Vol 1821, Issue 6, June 2012 DOI: 10.1016/j.bbalip.2012.02.007
URI: http://hdl.handle.net/10027/8535
ISSN: 0006-3002
Sponsor: This research was supported by NIH grants R01 HL68585 from NHLBI, and R21 DK78165 from NIDDK and the Office of dietary supplements (to PVS), and NIH grants RO1 HL093419 and R01 HL093419-01A1 (to JXC).
Date Available in INDIGO: 2012-08-17
 

This item appears in the following Collection(s)

Show full item record

Statistics

Country Code Views
United States of America 196
China 45
United Kingdom 9
France 7
Netherlands 4

Browse

My Account

Information

Access Key