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Hydrogen Peroxide Regulates Extracellular Superoxide Dismutase Activity and Expression in Neonatal Pulmonary Hypertension

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Title: Hydrogen Peroxide Regulates Extracellular Superoxide Dismutase Activity and Expression in Neonatal Pulmonary Hypertension
Author(s): Wedgwood, Stephen; Lakshminrusimha, Satyan; Fukai, Tohru; Russell, James A.; Schumacker, Paul T.; Steinhorn, Robin H.
Subject(s): cardiovascular disease in-vivo
Abstract: We previously demonstrated that superoxide and H(2)O(2) promote pulmonary arterial vasoconstriction in a lamb model of persistent pulmonary hypertension of the newborn (PPHN). Because extracellular superoxide dismutase (ecSOD) augments vasodilation, we hypothesized that H(2)O(2)-mediated ecSOD inactivation contributes to pulmonary arterial vasoconstriction in PPHN lambs. ecSOD activity was decreased in pulmonary arterial smooth muscle cells (PASMCs) isolated from PPHN lambs relative to controls. Exposure to 95% O(2) to mimic hyperoxic ventilation reduced ecSOD activity in control PASMCs. In both cases, these events were associated with increased protein thiol oxidation, as detected by the redox sensor roGFP. Accordingly, exogenous H(2)O(2) decreased ecSOD activity in control PASMCs, and PEG-catalase restored ecSOD activity in PPHN PASMCs. In intact animal studies, ecSOD activity was decreased in fetal PPHN lambs, and in PPHN lambs ventilated with 100% O(2) relative to controls. In ventilated PPHN lambs, administration of a single dose of intratracheal PEG-catalase enhanced ecSOD activity, reduced superoxide levels, and improved oxygenation. We propose that H(2)O(2) generated by PPHN and hyperoxia inactivates ecSOD, and intratracheal catalase enhances enzyme function. The associated decrease in extracellular superoxide augments vasodilation, suggesting that H(2)O(2) scavengers may represent an effective therapy in the clinical management of PPHN.
Issue Date: 2011-08-05
Publisher: Mary Ann Liebert
Citation Info: Wedgwood, S., Lakshminrusimha, S., Fukai, T., Russell, J. A., Schumacker, P. T., & Steinhorn, R. H. 2011. Hydrogen Peroxide Regulates Extracellular Superoxide Dismutase Activity and Expression in Neonatal Pulmonary Hypertension. Antioxidants & Redox Signaling, 15(6): 1497-1506. DOI: 10.1089/ars.2010.3630.
Type: Article
Description: This is a copy of an article published in the Antioxidants and Redox Signaling © 2011 [copyright Mary Ann Liebert, Inc.]; Antioxidants and Redox Signaling is available online at: http://www.liebertonline.com. DOI: 10.1089/ars.2010.3630.
URI: http://hdl.handle.net/10027/8566
ISSN: 1523-0864
Sponsor: These studies were supported by NIH grants HL54705 (R. H. S.), HL35440 and HL079650 (P. T. S.), and an American Heart Association Midwest Affiliate grant 0850137Z (S.W.).
Date Available in INDIGO: 2012-08-20
 

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