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Polycistronic coexpression and nondenaturing purification of histone octamers

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Title: Polycistronic coexpression and nondenaturing purification of histone octamers
Author(s): Shim, Yoonjung; Duan, Ming-Rui; Chen, Xuejing; Smerdon, Michael J.; Min, Jung-Hyun
Abstract: Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions. This approach reduces the time needed for the octamer preparation to a single day and should be applicable to making a variety of unmodified and modified histone octamers.
Issue Date: 2012-08-15
Publisher: Elsevier Masson
Citation Info: Shim Y, Duan MR, Chen X, Smerdon MJ, Min JH. Polycistronic coexpression and nondenaturing purification of histone octamers. Anal Biochem. 2012 Aug 15;427(2):190-2. DOI: 10.1016/j.ab.2012.05.006
Type: Article
Description: NOTICE: this is the author’s version of a work that was accepted for publication in Analytical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytical Biochemistry, [427 (2), (2012 Aug 15)] DOI: 10.1016/j.ab.2012.05.006
URI: http://hdl.handle.net/10027/8618
ISSN: 0003-269710.1016/j.ab.2012.05.006
Date Available in INDIGO: 2012-09-06
 

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