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The development of immunological tools as methods to investigate T cell dynamics.

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Title: The development of immunological tools as methods to investigate T cell dynamics.
Author(s): Jawaid, Omar
Advisor(s): Chen, Zheng
Department / Program: Microbiology/Immunology
Graduate Major: Microbiology/Immunology
Degree Granting Institution: University of Illinois at Chicago
Degree: MS, Master of Science
Genre: Masters
Subject(s): T cell gamma delta T cell Mycobacterium tuberculosis MHC-II tetramers Listeria monocytogenes vaccine vector IL-22 superantigen
Abstract: The investigation of T cell dynamics requires ever evolving tools in order to address questions and concerns. A protocol to reliably construct MHC-II tetramers is demonstrated as well as a method to deliver proteins intracellularly via a Listeria monocytogenes vaccine vector. These tools were then used to answer questions surrounding a hypothesized Mycobacterium tuberculosis superantigen as well as the dynamics of Vg2Vd2 T cells proliferation in vitro. While developing a T cell expansion assay, it was found that MHC-II tetramers complexed with TSST-1 or SEB were unable to proliferate T cells in vitro. This has implications on determining the full set of signals needed to proliferate T cells in vitro. Moreover, IL-22 delivered intracellularly to infected THP-1 macrophages was unable to proliferate gamma-delta T cells. This implies that IL-22 is unnecessary in proliferating gamma-delta T cells despite other evidence to the contrary.
Issue Date: 2012-12-10
Genre: thesis
Rights Information: Copyright 2012 Omar Jawaid
Date Available in INDIGO: 2012-12-10
Date Deposited: 2012-05

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