10027/20045
EX Du
EX
Du
XF Wang
XF
Wang
WC Yang
WC
Yang
D. Kaback
D.
Kaback
SP Yee
SP
Yee
CL Qin
CL
Qin
A. George
A.
George
JJ Hao
JJ
Hao
Characterization of Fam20C expression in odontogenesis and osteogenesis using transgenic mice.
University of Illinois at Chicago
2016
Fam20C
green fluorescence protein
green fluorescence protein report
mouse
promoter
2016-01-25 00:00:00
Journal contribution
https://indigo.uic.edu/articles/journal_contribution/Characterization_of_Fam20C_expression_in_odontogenesis_and_osteogenesis_using_transgenic_mice_/10763345
Our previous studies have demonstrated that Fam20C promotes differentiation and mineralization of odontoblasts, ameloblasts, osteoblasts and osteocytes during tooth and bone development. Ablation of the Fam20C gene inhibits bone and tooth growth by increasing fibroblast growth factor 23 in serum and causing hypophosphatemia in conditional knockout mice. However, control and regulation of the expression of Fam20C are still unknown. In this study, we generated a transgenic reporter model which expresses green fluorescence protein (GFP) driven by the Fam20C promoter. Recombineering was used to insert a 16 kb fragment of the mouse Fam20C gene (containing the 15 kb promoter and 1.1 kb of exon 1) into a pBluescript SK vector with the topaz variant of GFP and a bovine growth hormone polyadenylation sequence. GFP expression was subsequently evaluated by histomorphometry on cryosections from E14 to adult mice. Fluorescence was evident in the bone and teeth as early as E17.5. The GFP signal was maintained stably in odontoblasts and osteoblasts until 4 weeks after birth. The expression of GFP was significantly reduced in teeth, alveolar bone and muscle by 8 weeks of age. We also observed colocalization of the GFP signal with the Fam20C antibody in postnatal 1- and 7-day-old animals. Successful generation of Fam20C-GFP transgenic mice will provide a unique model for studying Fam20C gene expression and the biological function of this gene during odontogenesis and osteogenesis.