Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the 2 Neurotransmitter Glutamate In Vitro RountreeCM TroyJB SaggereL 2018 This protocol describes a novel method for investigating a form of chemical neurostimulation of wholemount rat retinas in vitro with the neurotransmitter glutamate. Chemical neurostimulation is a promising alternative to the conventional electrical neurostimulation of retinal neurons for treating irreversible blindness caused by photoreceptor degenerative diseases.