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Electrode Calibration with a Microfluidic Flow Cell for Fast-scan Cyclic Voltammetry

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journal contribution
posted on 2013-12-06, 00:00 authored by Elly Sinkala, James E. McCutcheon, Matt Schuck, Eric Schmidt, Mitchell F. Roitman, David T. Eddington
Fast-scan cyclic voltammetry (FSCV) is a common analytical electrochemistry tool used to measure chemical species. It has recently been adapted for measurement of neurotransmitters such as dopamine in awake and behaving animals (in vivo). Electrode calibration is an essential step in FSCV to relate observed current to concentration of a chemical species. However, existing methods require multiple components, which reduce the ease of calibrations. To this end, a microfluidic flow cell (μFC) was developed as a simple device to switch between buffer and buffer with a known concentration of the analyte of interest – in this case dopamine - in a microfluidic Y-channel. The ability to quickly switch solutions yielded electrode calibrations with faster rise times and that were more stable at peak current values. The μFC reduced the number of external electrical components and produced linear calibrations over a range of concentrations. To demonstrate this, an electrode calibrated with the μFC was used in FSCV recordings from a rat during the delivery of food reward – a stimulus that reliably evokes a brief increase in current due to the oxidation of dopamine. Using the linear calibration, dopamine concentrations were determined from the current responses evoked during the behavioral task. The μFC is able to easily and quickly calibrate FSCV electrode responses to chemical species for both in vitro and in vivo experiments.

Funding

Support for this work was provided by NIH MH085073 and NIH R01 DA025634 to MFR

History

Publisher Statement

Post print version of article may differ from published version. The definitive version is available through Royal Society of Chemistry at DOI: 10.1039/c2lc40168a

Publisher

Royal Society of Chemistry

Language

  • en_US

issn

1473-0197

Issue date

2012-07-01

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