posted on 2019-01-14, 00:00authored byRasha E. Shalaby, Saira Iram, Claudia E. Oropeza, Alan McLachlan
Transcriptional coactivators represent critical components of the transcriptional pre-initiation complex and are required for efficient gene activation. Members of the peroxisome proliferator-activated receptor gamma coactivator 1 (PGC1) family differentially regulate hepatitis b virus (HBV) biosynthesis. Whereas PGC1α has been shown to be a potent activator of HBV biosynthesis, PGC1β only very poorly activates HBV RNA and DNA synthesis in human hepatoma (HepG2) and embryonic kidney (HEK293T) cells. Furthermore, PGC1β inhibits PGC1α-mediated HBV biosynthesis. These observations suggest that a potential competition between human hepatoma (HepG2) and embryonic kidney (HEK293T) cells PGC1α and PGC1β for common transcription factor target(s) may regulate HBV transcription and replication in a context and signal transduction pathway dependent manner.
Funding
This work was supported by the National Institutes of Health (Grant number AI125401). We are grateful to Dr. Anastasia Kralli (The Scripps Research Institute, La Jolla, CA) for the plasmids pcDNA3-HA-hPGC1 pcDNA3-HA-hPGC1β and pSG5-HA-CBP, Dr. Malcolm G. Parker (Imperial Cancer Research Fund, London,
UK) for the plasmids pSG5-HA-SRC1e and pSG5-HA-GRIP1(SRC2) and Dr. Robert G. Roeder (The Rockefeller University, New York, NY) for the plasmids pIRES- FHneoP300, pIRESneoPRMT1 and pIRESneoCARM1(PRMT4).
History
Citation
Shalaby, R. E., Iram, S., Oropeza, C. E., & McLachlan, A. (2019). Peroxisome proliferator-activated receptor γ coactivator family members competitively regulate hepatitis b virus biosynthesis. Virology, 526, 214-221. doi:10.1016/j.virol.2018.10.027