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Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

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posted on 2022-10-21, 04:48 authored by Laudine MC Petralia, Esrath Santha, Anna-Janina Behrens, D Linh Nguyen, Mehul B Ganatra, Christopher H Taron, Vishal Khatri, Ramaswamy KalyanasundaramRamaswamy Kalyanasundaram, Angela van Diepen, Cornelis H Hokke, Jeremy M Foster

Serum N-glycan profiling studies during the past decades have shown robust associations between N-glycan changes and various biological conditions, including infections, in humans. Similar studies are scarcer for other mammals, despite the tremendous potential of serum N-glycans as biomarkers for infectious diseases in animal models of human disease and in the veterinary context. To expand the knowledge of serum N-glycan profiles in important mammalian model systems, in this study, we combined MALDI-TOF-MS analysis and HILIC-UPLC profiling of released N-glycans together with glycosidase treatments to characterize the glycan structures present in rhesus macaque serum. We used this baseline to monitor changes in serum N-glycans during infection with Brugia malayi, a parasitic nematode of humans responsible for lymphatic filariasis, in a longitudinal cohort of infected rhesus macaques. Alterations of the HILIC-UPLC profile, notably of abundant structures, became evident as early as 5 weeks post-infection. Given its prominent role in the immune response, contribution of immunoglobulin G to serum N-glycans was investigated. Finally, comparison with similar N-glycan profiling performed during infection with the dog heartworm Dirofilaria immitis suggests that many changes observed in rhesus macaque serum N-glycans are specific for lymphatic filariasis. 

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Petralia, Laudine, Esrath Santha, Anna-Janina Behrens, D. Linh Nguyen, Mehul B. Ganatra, Christopher H. Taron, Vishal Khatri et al. "Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi." Scientific Reports 12, no. 1 (2022): 1-16. https://doi.org/10.1038/s41598-022-19964-1

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Nature Research

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