University of Illinois at Chicago
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Glowing Koi of Cancer Cells

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posted on 2012-01-03, 00:00 authored by Cheng-Fen Chen
My project focused on the regulation of DNA Topoisomerase II (Top2) protein, one of the important targets for anticancer drugs. Top2 inhibitors can bind to Top2-DNA complex in cancer cells and inhibit their proliferation. The manipulation of Top2 expression in cancer cells will give us more insight into its role in cancer biology. I first transduced a shTop2 construct into the genome of MCF-7 breast cancer cells which can inhibit the generation of Top2 protein in the cells. Furthermore, I introduced a pLV-tTR-KRAB-Red construct into these modified cells which is an approach to turn on/ off Top2 gene expression into those shTop2modified breast cancer cells. In this image, MCF-7 breast cancer cells with integration of pLV-tTR-KRAB-Red construct emit red fluorescent and can be detected under the fluorescent microscope. These MCF-7 breast cancer cells were grown and maintained in cell culture dish and at the time I monitored the expression of pLV-tTR-KRAB-Red construct inside MCF-7 breast cancer cells, these cells happened to form this “Koi” shape by many cells. The dark circles represent the nucleus of each cell.


University of Illinois at Chicago Graduate College


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Entry 2011 in The Image of Research, a competition for students in graduate or professional degree programs at UIC, sponsored by UIC's Graduate College and the University Library. Images of award recipients and honorable mention images on exhibition in the Richard J. Daley Library, April 13-May 30, 2011.

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