Glowing Koi of Cancer Cells
figureposted on 03.01.2012 by Cheng-Fen Chen
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
My project focused on the regulation of DNA Topoisomerase II (Top2) protein, one of the important targets for anticancer drugs. Top2 inhibitors can bind to Top2-DNA complex in cancer cells and inhibit their proliferation. The manipulation of Top2 expression in cancer cells will give us more insight into its role in cancer biology. I first transduced a shTop2 construct into the genome of MCF-7 breast cancer cells which can inhibit the generation of Top2 protein in the cells. Furthermore, I introduced a pLV-tTR-KRAB-Red construct into these modified cells which is an approach to turn on/ off Top2 gene expression into those shTop2modified breast cancer cells. In this image, MCF-7 breast cancer cells with integration of pLV-tTR-KRAB-Red construct emit red fluorescent and can be detected under the fluorescent microscope. These MCF-7 breast cancer cells were grown and maintained in cell culture dish and at the time I monitored the expression of pLV-tTR-KRAB-Red construct inside MCF-7 breast cancer cells, these cells happened to form this “Koi” shape by many cells. The dark circles represent the nucleus of each cell.