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A microfluidic array for real-time live-cell imaging of human and rodent pancreatic islets.

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posted on 11.03.2017 by M Nourmohammadzadeh, Y Xing, JW Lee, MA Bochenek, JE Mendoza-Elias, JJ McGarrigle, E Marchese, Y Chun-Chieh, DT Eddington, J Oberholzer, Y Wang
In this study, we present a microfluidic array for high-resolution imaging of individual pancreatic islets. The device is based on hydrodynamic trapping principle and enables real-time analysis of islet cellular responses to insulin secretagogues. This device has significant advantages over our previously published perifusion chamber device including significantly increased analytical power and assay sensitivity, as well as improved spatiotemporal resolution. The islet array, with live-cell multiparametric imaging integration, provides a better tool to understand the physiological and pathophysiological changes of pancreatic islets through the analysis of single islet responses. This platform demonstrates the feasibility of array-based islet cellular analysis and opens up a new modality to conduct informative and quantitive evaluation of islets and cell-based screening for new diabetes treatments.

Funding

This work was in part supported by NIH R01 DK091526 (JO, YW), JDRF Microfluidic-Based Functional Facility at UIC, and the Chicago Diabetes Project (CDP).

History

Publisher Statement

This is a copy of an article published in Lab on a Chip © 2016 Royal Society of Chemistry Publications.

Publisher

Royal Society of Chemistry

Language

en_US

issn

1473-0197

Issue date

12/04/2016

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