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Cyclic strain dominates over microtopography in regulating cytoskeletal and focal adhesion remodeling of human mesenchymal stem cells

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journal contribution
posted on 19.11.2013 by Golnar Doroudian, Matthew W. Curtis, Anjulie Gang, Brenda Russell
Human bone marrow-derived mesenchymal stem cell (hMSCs) function depends on chemical factors and also on the physical cues of the microenvironmental niche. Here, this physical microenvironment is recapitulated with controlled modes of mechanical strain applied to substrata containing three-dimensional features in order to analyze the effects on cell morphology, focal adhesion distribution, and gene expression. 10% strain at 1 Hz is delivered for 48h to hMSCs cultured on flat surfaces, or on substrata with 15 μm-high microtopographic posts spaced 75 μm apart. Adding strain to microtopography produced stable semicircular focal adhesions, and actin spanning from post to post. Strain dominated over microtopography for expression of genes for the cytoskeleton (caldesmon-1 and calponin 3), cell adhesion (integrin-α2, vinculin, and paxillin), and extracellular matrix remodeling (MMP13) (P<0.05). Overall, attention to external mechanical stimuli is necessary for optimizing the stem cell niche for regenerative medicine.


This investigation was funded in part by the National Institutes of Health through grants HL090523 (BR) and T32 HL07692 (GD). Human MSCs were supplied under the auspices of an NIH grant 'Preparation and Distribution of Adult Stem Cells' (P 40 RR017447) to Darwin J. Prockop, M.D., Ph.D., from Texas A&M Health Science Center College of Medicine, Temple, TX.


Publisher Statement

NOTICE: This is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, Vol 430, Issue 3, (2013) DOI: 10.1016/j.bbrc.2012.11.120







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