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Diversity of the oral microbiome between dentate and edentulous individuals

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journal contribution
posted on 29.03.2019, 00:00 by Kyle Gazdeck, Sarah R. Fruscione, Guy R. Adami, Yalu Zhou, Lyndon F. Cooper, Joel L. Schwartz
Background: Measurement of saliva microbes is promoted as a way to detect oral and systemic disease, yet there is a multitude of factors that affect the oral microbiome. The salivary microbiome is influenced by oral biofilm of shedding (epithelial) and non‐shedding (tooth) surfaces. Methods: To gauge the ability of salivary microbial analytics to distinguish between edentulous and dentate oral conditions, we looked for differences in the saliva microbiome of subjects with and without teeth. 52 dentate and 49 edentulous subjects provided stimulated saliva samples. 16S rRNA gene sequencing, QIIME‐based data processing, and statistical analysis was done using several different analytical approaches to detect differences in the salivary microbiome between the two groups. Results: Bacteria diversity was lower in the edentulous group. Remarkably, all 31 of the most significant differences in taxa were deficits that occur in the edentulous group. As one might expect many of these taxa are attributed to dental plaque and gingival sulcus associated bacteria. Conclusion: In sum, the measurement of 16S rRNA genes in the bacteria of the saliva can be used to reproducibly measure differences in the oral microbiome that occur with edentulism, mainly the lack of tooth and tooth related structure

Funding

R.K. Gazdeck, L. Cooper, and J.L. Schwartz contributed to conception, design and data interpretation. G.R. Adami did the computational genomic analysis, data analysis and with R.K. Gazdek drafted the manuscript. L.F. Cooper and J. L. Schwartz critically revised the manuscript. S.R. Fruscione and Yalu Zhou did computational genomic analysis and data presentation. All authors gave final approval and agree to be accountable for all aspects of the work.

History

Publisher Statement

This is the pre-peer reviewed version of the following article: Gazdeck, R. K., Fruscione, S. R., Adami, G. R., Zhou, Y., Cooper, L. F., & Schwartz, J. L. (2019). Diversity of the oral microbiome between dentate and edentulous individuals. Oral Diseases, which has been published in final form at 10.1111/odi.13039,

Citation

Gazdeck, R. K., Fruscione, S. R., Adami, G. R., Zhou, Y., Cooper, L. F., & Schwartz, J. L. (2019). Diversity of the oral microbiome between dentate and edentulous individuals. Oral Diseases. doi:10.1111/odi.13039

Publisher

Wiley

Language

en_US

issn

1354-523X

Issue date

10/02/2019

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