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Expression of the G72/G30 gene in transgenic mice induces behavioral changes

journal contribution
posted on 29.03.2016 by Lijun Cheng, Eiji Hattori, Akira Nakajima, Nancy Woehrle, Mark D. Opal, Chunling Zhang, Kay Grennan, Stephanie C. Dulawa, Ya-Ping Tang, Elliot S. Gershon, Chunyu Liu
The G72/G30 gene complex is a candidate gene for schizophrenia and bipolar disorder. However, G72 and G30 mRNAs are expressed at very low levels in human brain, with only rare splicing forms observed. We report here G72/G30 expression profiles and behavioral changes in a G72/G30 transgenic mouse model. A human BAC clone containing the G72/G30 genomic region was used to establish the transgenic mouse model, on which gene expression studies, Western blot and behavioral tests were performed. Relative to their minimal expression in humans, G72 and G30 mRNAs were highly expressed in the transgenic mice, and had a more complex splicing pattern. The highest G72 transcript levels were found in testis, followed by cerebral cortex, with very low or undetectable levels in other tissues. No LG72 (the long putative isoform of G72) protein was detected in the transgenic mice. Whole-genome expression profiling identified 361 genes differentially-expressed in transgenic mice compared to wild-type, including genes previously implicated in neurological and psychological disorders. Relative to wild-type mice, the transgenic mice exhibited fewer stereotypic movements in the open field test, higher baseline startle responses in the course of the prepulse inhibition test, and lower hedonic responses in the sucrose preference test. The transcriptome profile changes and multiple mouse behavioral effects suggest that the G72 gene may play a role in modulating behaviors relevant to psychiatric disorders.

Funding

This work was supported by 1R21MH083521 and 4R33MH083521 (to CL).

History

Publisher Statement

© 2013 by Nature Publishing Group, Molecular Psychiatry. Post print version of article may differ from published version. The definitive version is available through Nature Publishing Group at DOI:10.1038/mp.2012.185.

Publisher

Nature Publishing Group

Language

en_US

issn

1476-5578

Issue date

01/01/2013

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