pone.0009168.pdf (1.59 MB)
0/0

Posttranslational modifications, localization, and protein interactions of optineurin, the product of a glaucoma gene

Download (1.59 MB)
journal contribution
posted on 27.05.2011 by Hongyu Ying, Xiang Shen, BumChan Park, Beatrice Y. J. T. Yue
Background: Glaucoma is a major blinding disease. The most common form of this disease, primary open angle glaucoma (POAG), is genetically heterogeneous. One of the candidate genes, optineurin, is linked principally to normal tension glaucoma, a subtype of POAG. The present study was undertaken to illustrate the basic characteristics of optineurin. Methodology/Principal Findings: Lysates from rat retinal ganglion RGC5 cells were subjected to N- or O-deglycosylation or membrane protein extraction. The phosphorylation status was evaluated after immunoprecipitation. It was found that while phosphorylated, optineurin was neither N- nor O-glycosylated, and was by itself not a membrane protein. RGC5 and human retinal pigment epithelial cells were double stained with anti-optineurin and anti-GM130. The endogenous optineurin exhibited a diffuse, cytoplasmic distribution, but a population of the protein was associated with the Golgi apparatus. Turnover experiments showed that the endogenous optineurin was relatively short-lived, with a half-life of approximately 8 hours. Native blue gel electrophoresis revealed that the endogenous optineurin formed homohexamers. Optineurin also interacted with molecules including Rab8, myosin VI, and transferrin receptor to assemble into supermolecular complexes. When overexpressed, optineurin–green fluorescence protein (GFP) fusion protein formed punctate structures termed ‘‘foci’’ in the perinuclear region. Treatment of nocadazole resulted in dispersion of the optineurin foci. In addition, tetracyclineregulated optineurin-GFPs expressing RGC5 stable cell lines were established for the first time. Conclusions/Significance: The present study provides new information regarding basic characteristics of optineurin that are important for future efforts in defining precisely how optineurin functions normally and how mutations may result in pathology. The inducible optineurin-GFP–expressing cell lines are also anticipated to facilitate in-depth studies of optineurin. Furthermore, the demonstrations that optineurin is an aggregation-prone protein and that the foci formation is microtubule-dependent bear similarities to features documented in neurodegenerative diseases, supporting a neurodegenerative paradigm for glaucoma.

Funding

This work was supported by grants EY018828, EY005628, and EY003890 (to B.Y.J.T.Y.); by Core Grant EY01792 from the National Eye Institute, National Institutes of Health (www.nei.nih.gov; Bethesda, Maryland); and by a grant from the American Health Assistance Foundation (www.ahaf.org; Clarksburg, Maryland).

History

Publisher Statement

2010 Ying et al. This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. DOI: 10.1371/journal.pone.0009168

Publisher

Public Library of Science

Language

en_US

issn

1932-6203

Issue date

11/02/2010

Exports

Categories

Exports