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Pseudomonas aeruginosa induced lung injury model.

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journal contribution
posted on 04.05.2016 by V. Suresh Kumar, R.T. Sadikot, J.E. Purcell, A.B. Malik, Y. Liu
In order to study human acute lung injury and pneumonia, it is important to develop animal models to mimic various pathological features of this disease. Here we have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa (P. aeruginosa or PA). Using this model, we were able to show lung inflammation at the early phase of injury. In addition, alveolar epithelial barrier leakiness was observed by analyzing bronchoalveolar lavage (BAL); and alveolar cell death was observed by Tunel assay using tissue prepared from injured lungs. At a later phase following injury, we observed cell proliferation required for the repair process. The injury was resolved 7 days from the initiation of P. aeruginosa injection. This model mimics the sequential course of lung inflammation, injury and repair during pneumonia. This clinically relevant animal model is suitable for studying pathology, mechanism of repair, following acute lung injury, and also can be used to test potential therapeutic agents for this disease.

Funding

This work was supported by National Institutes of Health grants HL105947-01 (YL), HL07829-16 (AM), HL090152 (AM)

History

Publisher Statement

This is a copy of an article published in the Journal of Visualized Experiments © 2014 Journal of Visualized Experiments Publications.

Publisher

Journal of Visualized Experiments

Issue date

29/10/2014

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