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The use of proteomics studies in identifying moonlighting proteins

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journal contribution
posted on 16.11.2018 by Constance Jeffery
Proteomics studies that characterize hundreds or thousands of proteins in parallel can play an important part in the identification of moonlighting proteins, proteins that perform two or more distinct and physiologically relevant biochemical or biophysical functions. Functional assays, including ligand binding assays, can find a surprising second function for a protein that was previously identified as performing a different function, for example, a DNA binding ability for an enzyme in amino acid metabolism. The results of large scale assays of protein-protein interactions, gene knockouts, or subcellular protein localizations, or bioinformatics analysis of amino acid sequences and three-dimensional structures, can also be used to predict that a protein has additional functions, but in these cases it is important to use biochemical and biophysical methods to confirm the protein can perform each function.

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Publisher Statement

Copyright @ Humana Press

Citation

Jeffery, C. (2019). The Use of Proteomics Studies in Identifying Moonlighting Proteins. Methods in molecular biology (Clifton, N.J.), 1871, 437-443. doi:10.1007/978-1-4939-8814-3_25

Publisher

Humana Press

Language

en_US

issn

1064-3745

Issue date

02/10/2018

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