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Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons

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posted on 27.05.2022, 18:42 authored by Yi-Wen Hsieh, Chiou-Fen ChuangChiou-Fen Chuang
The Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC development using an integrated unc-62::GFP fosmid rescuing transgene. UNC-62::GFP was not detected in AWC neurons in early or late embryos. These results are consistent with previous single-cell RNA sequencing data and also suggest an undetectable level of unc-62 expression and/or low stability of UNC-62 protein in AWC neurons during embryogenesis.

Funding

Specification of Stochastic Left-Right Asymmetric Neuronal Fates in C. Elegans | Funder: National Institutes of Health (National Institute of General Medical Sciences) | Grant ID: R01GM098026

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Hsieh, Y. -W.Chuang, C. -F. (2022). Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons. microPublication Biology, 2022, 10.17912/micropub.biology.000530-. https://doi.org/10.17912/micropub.biology.000530

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en

issn

2578-9430

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