unc-62-GFP expression_microPublication Biology_2_18_22.pdf (3.76 MB)
Download fileAnalysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
journal contribution
posted on 2022-05-27, 18:42 authored by Yi-Wen Hsieh, Chiou-Fen ChuangChiou-Fen ChuangThe Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC development using an integrated unc-62::GFP fosmid rescuing transgene. UNC-62::GFP was not detected in AWC neurons in early or late embryos. These results are consistent with previous single-cell RNA sequencing data and also suggest an undetectable level of unc-62 expression and/or low stability of UNC-62 protein in AWC neurons during embryogenesis.
Funding
Specification of Stochastic Left-Right Asymmetric Neuronal Fates in C. Elegans | Funder: National Institutes of Health (National Institute of General Medical Sciences) | Grant ID: R01GM098026
History
Citation
Hsieh, Y. -W.Chuang, C. -F. (2022). Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons. microPublication Biology, 2022, 10.17912/micropub.biology.000530-. https://doi.org/10.17912/micropub.biology.000530Language
- en