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Basal Autophagy Is Required for Herpes simplex Virus-2 Infection.

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journal contribution
posted on 27.01.2016, 00:00 by AM Yakoub, D. Shukla
Autophagy is a conserved catabolic process of the cell, which plays an important role in regulating plethora of infections. The role of autophagy in Herpes simplex virus-2 (HSV-2) infection is unknown. Here, we found that HSV-2 does not allow induction of an autophagic response to infection, but maintains basal autophagy levels mostly unchanged during productive infection. Thus, we investigated the importance of basal autophagy for HSV-2 infection, using pharmacological autophagy suppression or cells genetically deficient in an autophagy-essential gene (ATG5). Interference with basal autophagy flux in cells significantly reduced viral replication and diminished the infection. These results indicate that basal autophagy plays an indispensable role required for a productive infection. Importantly, this study draws a sharp distinction between induced and basal autophagy, where the former acts as a viral clearance mechanism abrogating infection, while the latter supports infection.

Funding

This study was supported by a NIH grant (EY023058) to D.S. and a core grant (EY01792). The authors claim no conflicts of interest.

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Publisher Statement

This is the copy of an article published in Scientific Reports © 2015 Nature Publishing Group Publications. © The Author(s).

Publisher

Nature Publishing Group

issn

2045-2322

Issue date

07/08/2015

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