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Carboxypeptidase M augments kinin B1 receptor signaling by conformational crosstalk and enhances endothelial nitric oxide output

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journal contribution
posted on 15.04.2014, 00:00 authored by Xianming Zhang, Fulong Tan, Viktor Brovkovych, Yongkang Zhang, Jessica L. Lowry, Randal A. Skidgel
The G protein-coupled receptors (GPCRs) are the largest class of membrane proteins that play key roles in transducing extracellular signals to intracellular proteins to generate cellular responses. The kinin GPCRs, named B1 (B1R) and B2 (B2R), are responsible for mediating the biological responses to kinin peptides released from the precursor kininogens. Bradykinin (BK) or kallidin (KD) are agonists for B2Rs, whereas their carboxypeptidase (CP)-generated metabolites, des-Arg(9)-BK or des-Arg(10)-KD, are specific agonists for B1Rs. Here, we review the evidence for a critical role of membrane-bound CPM in facilitating B1R signaling by its ability to directly activate the receptor via conformational crosstalk as well as generate its specific agonist. In endothelial cells, the CPM/B1R interaction facilitates B1R-dependent high-output nitric oxide under inflammatory conditions.

Funding

This work was supported by the National Institutes of Health Grants DK41431 and HL60678

History

Publisher Statement

This is a copy of an article published in the Biological Chemistry © 2013 Walter de Gruyter. The final publication is available at http://www.degruyter.com/view/j/bchm doi: 10.1515/hsz-2012-0290.

Publisher

Walter de Gruyter

Language

en_US

issn

1431-6730

Issue date

01/03/2013

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