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Characterization of the yehUT Two-Component Regulatory System of Salmonella enterica Serovar Typhi and Typhimurium

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posted on 2016-04-05, 00:00 authored by Vanessa K Wong, Derek J Pickard, Lars Barquist, Karthikeyan Sivaraman, Andrew J Page, Peter J Hart, Mark J Arends, Kathryn E Holt, Leanne Kane, Lynda F Mottram, Louise Ellison, Ruben Bautista, Chris J McGee, Sally J Kay, Thomas M Wileman, Linda J Kenney, Calman A Maclennan, Robert A Kingsley, Gordon Dougan
Proteins exhibiting hyper-variable sequences within a bacterial pathogen may be associated with host adaptation. Several lineages of the monophyletic pathogen Salmonella enterica serovar Typhi (S. Typhi) have accumulated non-synonymous mutations in the putative two-component regulatory system yehUT. Consequently we evaluated the function of yehUT in S. Typhi BRD948 and S. Typhimurium ST4/74. Transcriptome analysis identified the cstA gene, encoding a carbon starvation protein as the predominantly yehUT regulated gene in both these serovars. Deletion of yehUT had no detectable effect on the ability of these mutant Salmonella to invade cultured epithelial cells (S. Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes.

Funding

Wellcome Trust (Grant no: 09805; http://www.wellcome.ac.uk). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Publisher Statement

Copyright: © 2013 Wong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publisher

Public Library of Science

Language

  • en_US

issn

1932-6203

Issue date

2014-01-05

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