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Genome-wide effects of the antimicrobial peptide apidaecin on translation termination in bacteria

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journal contribution
posted on 28.04.2021, 17:14 by K Mangano, T Florin, X Shao, D Klepacki, I Chelysheva, Z Ignatova, Yu Gao, Alexander Mankin, N Vázquez-Laslop
Biochemical studies suggested that the antimicrobial peptide apidaecin (Api) inhibits protein synthesis by binding in the nascent peptide exit tunnel and trapping the release factor associated with a terminating ribosome. The mode of Api action in bacterial cells had remained unknown. Here genome-wide analysis reveals that in bacteria, Api arrests translating ribosomes at stop codons and causes pronounced queuing of the trailing ribosomes. By sequestering the available release factors, Api promotes pervasive stop codon bypass, leading to the expression of proteins with C-terminal extensions. Api-mediated translation arrest leads to the futile activation of the ribosome rescue systems. Understanding the unique mechanism of Api action in living cells may facilitate the development of new medicines and research tools for genome exploration.

Funding

Research Training in Natural Product Complementary and Integrative Health

National Center for Complementary and Integrative Health

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Highly sensitive proteomics method to probe cell heterogeneity at single cell resolution

National Institute of General Medical Sciences

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Control of translation by the nascent protein after its full synthesis and release

Directorate for Biological Sciences

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History

Citation

Mangano, K., Florin, T., Shao, X., Klepacki, D., Chelysheva, I., Ignatova, Z., Gao, Y., Mankin, A. S.Vázquez-Laslop, N. (2020). Genome-wide effects of the antimicrobial peptide apidaecin on translation termination in bacteria. eLife, 9, 1-24. https://doi.org/10.7554/eLife.62655

Publisher

eLife Sciences Publications, Ltd

Language

en

issn

2050-084X

Licence

Exports