Wang et al. 2022.pdf (5.36 MB)
Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery
journal contribution
posted on 2023-05-15, 16:50 authored by Xin Wang, Chih-Yu Pai, David StoneDavid StoneThe mating of budding yeast depends on chemotropism, a fundamental cellular process. Haploid yeast cells of opposite mating type signal their positions to one another through mating pheromones. We have proposed a deterministic gradient sensing model that explains how these cells orient toward their mating partners. Using the cell-cycle determined default polarity site (DS), cells assemble a gradient tracking machine (GTM) composed of signaling, polarity, and trafficking proteins. After assembly, the GTM redistributes up the gradient, aligns with the pheromone source, and triggers polarized growth toward the partner. Since positive feedback mechanisms drive polarized growth at the DS, it is unclear how the GTM is released for tracking. What prevents the GTM from triggering polarized growth at the DS? Here, we describe two mechanisms that are essential for tracking: inactivation of the Ras GTPase Bud1 and positioning of actin-independent vesicle delivery upgradient.
Funding
How yeast sense direction in shallow pheromone gradients | Funder: National Science Foundation | Grant ID: MCB-1818067
History
Citation
Wang, X., Pai, C. -Y.Stone, D. E. (2022). Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery. Journal of Cell Biology, 221(12), e202203004-. https://doi.org/10.1083/jcb.202203004Publisher
Rockefeller University PressLanguage
- en
issn
0021-9525Usage metrics
Categories
No categories selectedKeywords
3101 Biochemistry and Cell Biology31 Biological Sciences1 Underpinning research1.1 Normal biological development and functioningGeneric health relevanceActinsCell PolarityPheromonesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTransport Vesiclesrab GTP-Binding Proteinsras ProteinsDevelopmental Biology31 Biological sciences32 Biomedical and clinical sciencesBiological SciencesMedical and Health Sciences