HPLC-MTT assay: anti-cancer activity of aqueous garlic extract is from allicin
journal contribution
posted on 2014-01-03, 00:00 authored by Jenny Lee, Shalini Gupta, Jin-Sheng Huang, Lasanthi P. Jayathilaka, Bao-Shiang LeeA strategy using RP-HPLC, TLC, MS, NMR, chemical synthesis, and MTT cell viability assay to identify allicin as the active anti-cancer compound in the aqueous extract of garlic (AGE) is described. Change the pH of the AGE from 7 to 5 eliminated interfering molecules and enabled a clean HPLC separation of the constituents in AGE. MTT assay of the HPLC fractions identify an active fraction. Further analysis by TLC, MS, and NMR verify the active HPLC fraction as allicin. Chemically synthesized allicin is used to provide further confirmation. The results clearly identify the active compound in AGE as allicin.
History
Publisher Statement
NOTICE: This is the author’s version of a work that was accepted for publication in Analytical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytical Biochemistry, Vol 436, Issue 2, 2013 DOI:10.1016/j.ab.2013.01.033Publisher
Elsevier MassonLanguage
- en_US
issn
0003-2697Issue date
2013-05-01Usage metrics
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