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Hydrolyzed Ce(IV) salts limit sucrose-dependent biofilm formation by Streptococcus mutans

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posted on 2023-05-16, 16:34 authored by Lopa Bhatt, Lin ChenLin Chen, Jinglong Guo, Robert KlieRobert Klie, Junhe Shi, Russell PesaventoRussell Pesavento
Several studies have focused on the antimicrobial effects of cerium oxide nanoparticles (CeO2-NP) but few have focused on their effects on bacteria under initial biofilm formation conditions. Streptococcus mutans is a prolific biofilm former contributing to dental caries in the presence of fermentable carbohydrates and is a recognized target for therapeutic intervention. CeO2-NP derived solely from Ce(IV) salt hydrolysis were found to reduce adherent bacteria by approximately 40% while commercial dispersions of "bare" CeO2-NP (e.g., 3 nm, 10-20 nm, 30 nm diameter) and Ce(NO3)3·6H2O were either inactive or observed to slightly increase biofilm formation under similar in vitro conditions. Planktonic growth and dispersal assays support a non-bactericidal mode of biofilm inhibition active in the initial phases of S. mutans biofilm production. Human cell proliferation assays suggest only minor effects of hydrolyzed Ce(IV) salts on cellular metabolism at concentrations up to 1 mM Ce, with less observed toxicity compared to equimolar concentrations of AgNO3. The results presented herein have implications in clinical dentistry.

Funding

Multidisciplinary Oral Sciences Training Program | Funder: National Institutes of Health (National Institute of Dental and Craniofacial Research) | Grant ID: T32DE018381

MR1-R2: Acquisition Of An Aberration-Corrected Scanning Transmission Electron Microscope For Multidisciplinary Research And Education at UIC | Funder: National Science Foundation | Grant ID: DMR-0959470

MRI: Acquisition of Dual-EELS gatan Quantum Imaging Spectrometer to Upgrade the JEOL ARM200CF at UIC | Funder: National Science Foundation | Grant ID: DMR-1626065

Novel Biofilm Inhibitors of Oral Streptococci | Funder: National Institutes of Health (National Institute of Dental and Craniofacial Research) | Grant ID: K08DE028009

History

Citation

Bhatt, L., Chen, L., Guo, J., Klie, R. F., Shi, J.Pesavento, R. P. (2020). Hydrolyzed Ce(IV) salts limit sucrose-dependent biofilm formation by Streptococcus mutans. Journal of Inorganic Biochemistry, 206, 110997-. https://doi.org/10.1016/j.jinorgbio.2020.110997

Publisher

Elsevier

Language

  • en

issn

0162-0134