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Independent Activation of Hepatitis B Virus Biosynthesis by Retinoids, Peroxisome Proliferators and Bile Acids.

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journal contribution
posted on 03.12.2013, 00:00 by Vanessa C. Reese, Claudia E. Oropeza, Alan McLachlan
In the human hepatoma cell line HepG2, retinoic acid, clofibric acid, and bile acid treatment can only modestly increase hepatitis B virus (HBV) biosynthesis. Utilizing the human embryonic kidney cell line 293T, it was possible to demonstrate that the retinoid X receptor α (RXRα) plus its ligand can support viral biosynthesis independently of additional nuclear receptors. In addition, RXRα/peroxisome proliferator-activated receptor α (PPARα) and RXRα/farnesoid X receptor α (FXRα) heterodimeric nuclear receptors can also mediate ligand-dependent HBV transcription and replication when activated by clofibric acid and bile acid, respectively, independently of a requirement for the ligand-dependent activation of RXRα. These observations indicate that there are at least three possible modes of ligand-mediated activation of HBV transcription and replication existing within hepatocytes, suggesting that multiple independent mechanisms control viral production in the livers of infected individuals.

Funding

This work was supported by Public Health Service grant AI30070 and Ruth L. Kirschstein National Research Service Award AI081451 from the National Institutes of Health.

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Publisher Statement

Post print version of article may differ from published version. The definitive version is available through American Society for Microbiology at DOI:10.1128/JVI.01562-12

Publisher

American Society for Microbiology

Language

en_US

issn

0022-538X

Issue date

01/01/2013

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