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Knockout of the gene encoding the extracellular matrix protein SNED1 results in early neonatal lethality and craniofacial malformations
journal contributionposted on 04.11.2022, 21:48 authored by Anna Barqué, Kyleen Jan, Emanuel De La Fuente, Christina NicholasChristina Nicholas, Richard O Hynes, Alexandra NabaAlexandra Naba
BACKGROUND: The extracellular matrix (ECM) is a fundamental component of multicellular organisms that orchestrates developmental processes and controls cell and tissue organization. We previously identified the novel ECM protein SNED1 as a promoter of breast cancer metastasis and showed that its level of expression negatively correlated with breast cancer patient survival. Here, we sought to identify the roles of SNED1 during murine development. RESULTS: We generated two novel Sned1 knockout mouse strains and showed that Sned1 is essential since homozygous ablation of the gene led to early neonatal lethality. Phenotypic analysis of the surviving knockout mice revealed a role for SNED1 in the development of craniofacial and skeletal structures since Sned1 knockout resulted in growth defects, nasal cavity occlusion, and craniofacial malformations. Sned1 is widely expressed in embryos, notably by cell populations undergoing epithelial-to-mesenchymal transition, such as the neural crest cells. We further show that mice with a neural-crest-cell-specific deletion of Sned1 survive, but display facial anomalies partly phenocopying the global knockout mice. CONCLUSIONS: Our results demonstrate requisite roles for SNED1 during development and neonatal survival. Importantly, the deletion of 2q37.3 in humans, a region that includes the SNED1 locus, has been associated with facial dysmorphism and short stature.
CitationBarqué, A., Jan, K., De La Fuente, E., Nicholas, C. L., Hynes, R. O.Naba, A. (2020). Knockout of the gene encoding the extracellular matrix protein SNED1 results in early neonatal lethality and craniofacial malformations. Developmental Dynamics, 250(2), 274-294. https://doi.org/10.1002/dvdy.258
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PediatricCongenital Structural AnomaliesGeneticsBreast CancerPediatric Research InitiativePreventionCancerDental/Oral and Craniofacial DiseaseBiotechnology2.1 Biological and endogenous factors1.1 Normal biological development and functioningcraniofacial featuresgeometric morphometricsknockout mousemandibleneural crest cellsAmino Acid SequenceAnimalsConserved SequenceCraniofacial AbnormalitiesExtracellular Matrix ProteinsGenes, LethalGrowth DisordersMandibleMiceMice, KnockoutNasal CavityDevelopmental BiologyBiological SciencesMedical and Health Sciences