Lack of Vitamin D Receptor Leads to Hyperfunction of Claudin-2 in Intestinal Inflammatory Responses

Background: Vitamin D3 and vitamin D receptor (VDR) are involved in pathogenesis of inflammatory bowel disease (IBD) and bacterial infection. Claudin-2 is a junction protein that mediates paracellular water transport in epithelia. Elevation of Claudin-2 is associated with active IBD. However, VDR involved in epithelial junctions under inflammation and infection remains largely unknown. We investigated the mechanisms on how VDR and Claudin-2 are related in inflamed states. Methods: Using cultured VDR-/- enteroids, human intestinal epithelial cells, and VDR-/- mice with Salmonella- or DSS- colitis, and human IBD samples, we investigated the mechanisms how VDR and Claudin-2 are related in inflamed states. Results: After Salmonella-infection, we observed significantly enhanced Claudin-2 and an increased bacterial invasion and translocation. Lacking of VDR regulation led to a robust increase of Claudin-2 at the mRNA and protein levels post infection. In DSS-treated VDR-/- mice, Claudin-2 was significantly increased. Location and quantification of Claudin-2 protein in the mouse colons treated with DSS also confirmed these results. Inflammatory cytokines were significantly higher in the serum and mRNA levels in intestine, which are known to increase Claudin-2. Furthermore, in inflamed intestine of ulcerative colitis patients, VDR expression was low and Claudin-2 was enhanced. Mechanistically, we identified the enhanced Claudin-2 promoter activity through the binding sites of NF-κB and STAT in inflamed VDR-/- cells. Conclusions: Our studies have identified a new role for intestinal epithelial VDR in regulating barrier functions in the context of infection and inflammation.