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Linkage of Type I Interferon Activity and TNF-Alpha Levels in Serum with Sarcoidosis Manifestations and Ancestry

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posted on 07.08.2012, 00:00 by Nadera J. Sweiss, Wei Zhang, Beverly S. Franek, Silvia N. Kariuki, David R. Moller, Karen C. Patterson, Peggy Bennett, Lakshmi R. Girijala, Vaisak Nair, Robert P. Baughman, Joe G. N. Garcia, Timothy B. Niewold
Background: Both type I interferon (IFN), also known as IFN-a and tumor necrosis factor alpha (TNF-a) have been implicated in the pathogenesis of sarcoidosis. We investigated serum levels of these cytokines in a large multi-ancestral sarcoidosis population to determine correlations between cytokine levels and disease phenotypes. Methods: We studied serum samples from 98 patients with sarcoidosis, including 71 patients of African-American ancestry and 27 patients of European-American ancestry. Serum type I IFN was measured using a sensitive reporter cell assay and serum TNF-a was measured using a commercial ELISA kit. Clinical data including presence or absence of neurologic, cardiac, and severe pulmonary manifestations of sarcoidosis were abstracted from medical records. Twenty age-matched nonautoimmune controls were also studied from each ancestral background. Differences in cytokine levels between groups were analyzed with Mann-Whitney U test, and correlations were assessed using Spearman’s rho. Multivariate logistic regression models were used to detect associations between cytokines and clinical manifestations. Results: Significant differences in cytokine levels were observed between African- and European-American patients with sarcoidosis. In African-Americans, serum TNF-a levels were significantly higher relative to matched controls (P = 0.039), and patients with neurologic disease had significantly higher TNF-a than patients lacking this anifestation (P = 0.022). In European-Americans, serum type I IFN activity was higher in sarcoidosis cases as compared to matched controls, and patients with extra-pulmonary disease represented a high serum IFN subgroup (P = 0.0032). None of the associations observed were shared between the two ancestral groups. Conclusions: Our data indicate that significant associations between serum levels of TNF-a and type I IFN and clinical manifestations exist in a sarcoidosis cohort that differ significantly by self-reported ancestry. In each ancestral background, the cytokine elevated in patients with sarcoidosis was also associated with a particular disease phenotype. These findings may relate to ancestral differences in the molecular pathogenesis of this heterogeneous disease.

Funding

A Foundation for Sarcoidosis Research Young Investigators Award given to Nadera J. Sweiss was used to fund this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Publisher Statement

© 2011 Sweiss et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. doi:10.1371/journal.pone.0029126

Publisher

Public Library of Science

Language

en_US

issn

1932-6203

Issue date

14/12/2011

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