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Low-force DNA condensation and discontinuous high-force decondensation reveal a loop-stabilizing function of the protein Fis

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journal contribution
posted on 08.08.2009, 00:00 by Dunja Skoko, Jie Yan, Reid C. Johnson, John F. Marko
We report single-DNA-stretching experiments showing that the protein Fis, an abundant bacterial chromosome protein of E. coli, mediates a dramatic DNA condensation to zero length. This condensation occurs abruptly when DNA tension is reduced below a protein-concentration-dependent threshold f(*)< 1 pN. Following condensation, reopening under larger forces proceeds via a series of discrete jumps, indicating that Fis is able to stabilize DNA crossings. Our experiments suggest that Fis may play a role in vivo stabilizing the "loop-domain" structure of the bacterial chromosome.

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Publisher's Copyright: http://forms.aps.org/author/copytrnsfr.pdf

Publisher

American Physical Society

issn

0031-9007

Issue date

11/11/2005

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