Albeanu_2018.pdf (5.94 MB)
Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map
journal contributionposted on 2023-04-17, 22:21 authored by Dinu F Albeanu, Allison C Provost, Prateek Agarwal, Edward R Soucy, Joseph D Zak, Venkatesh N Murthy
Inputs from olfactory sensory neuron (OSN) axons expressing the same type of odorant receptor (OR) converge in the glomerulus of the main olfactory bulb. A key marker of mature OSNs is olfactory marker protein (OMP), whose deletion has been associated with deficits in OSN signal transduction and odor discrimination. Here, we investigate glomerular odor responses and anatomical architecture in mice in which one or both alleles of OMP are replaced by the fluorescent synaptic activity reporter, synaptopHluorin. Functionally heterogeneous glomeruli, that is, ones with microdomains with distinct odor responses, are rare in OMP+/- mice, but occur frequently in OMP-/- mice. Genetic targeting of single ORs reveals that these microdomains arise from co-innervation of individual glomeruli by OSNs expressing different ORs. This glomerular mistargeting is locally restricted to a few glomerular diameters. Our studies document functional heterogeneity in sensory input within individual glomeruli and uncover its anatomical correlate, revealing an unexpected role for OMP in the formation and refinement of the glomerular map.
Information Coding in Individual Olfactory Sensory Axons | Funder: National Institute on Deafness and Other Communication Disorders | Grant ID: F32DC015938
CitationAlbeanu, D. F., Provost, A. C., Agarwal, P., Soucy, E. R., Zak, J. D.Murthy, V. N. (2018). Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map. Nature Communications, 9(1), 5073-. https://doi.org/10.1038/s41467-018-07544-9
PublisherSpringer Science and Business Media LLC