Polycistronic coexpression and nondenaturing purification of histone octamers
journal contribution
posted on 2012-09-06, 00:00 authored by Yoonjung Shim, Ming-Rui Duan, Xuejing Chen, Michael J. Smerdon, Jung-Hyun MinHistone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions. This approach reduces the time needed for the octamer preparation to a single day and should be applicable to making a variety of unmodified and modified histone octamers.
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Publisher Statement
NOTICE: this is the author’s version of a work that was accepted for publication in Analytical Biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Analytical Biochemistry, [427 (2), (2012 Aug 15)] DOI: 10.1016/j.ab.2012.05.006Publisher
Elsevier Massonissn
0003-2697Issue date
2012-08-15Usage metrics
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