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Synthesis and characterization of DNA-quantum dot conjugates for the fluorescent ratiometric detection of unlabelled DNA

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journal contribution
posted on 18.12.2017, 00:00 authored by P. T. Snee, C.-T. Ho, C. M. Tyrakowski, X. Zhang, L. E. Page
A quantum dot-based ratiometrically responsive fluorescent sensor for unlabeled single-stranded DNA (ssDNA) is reported. Several technical issues concerning the development of high yield ssDNA-QD conjugation chemistry were addressed. The DNA sensor was synthesized by conjugating methacrylic phosphoramidite-functional oligonucleotides to water-soluble cadmium zinc sulfide core / zinc sulfide shell quantum dots (CdZnS/ZnS QDs). Duplex DNA was formed when the QD-bound ssDNA was incubated with its compliment. Next, titration with PicoGreen resulted in FRET energy is transfer from the dot to the dsDNA intercalating dye. The resulting ratio of the dye to QD integrated emissions is a calibratable metric for label-free DNA detection with a LOD of 3.8 nmol.

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Post print version of article may differ from published version. The definitive version is available through Royal Society of Chemistry at DOI:10.1039/c6an01760f

Publisher

Royal Society of Chemistry

issn

00032654

Issue date

22/11/2016

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