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TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis

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posted on 10.12.2021, 18:02 by ZJ Jiang, W Li, LH Yao, B Saed, Y Rao, BS Grewe, Andrea McGinleyAndrea McGinley, K Varga, Simon AlfordSimon Alford, Ying HuYing Hu, Liang-Wei GongLiang-Wei Gong
TRPM7 contributes to a variety of physiological and pathological processes in many tissues and cells. With a widespread distribution in the nervous system, TRPM7 is involved in animal behaviors and neuronal death induced by ischemia. However, the physiological role of TRPM7 in CNS neuron remains unclear. Here, we identify endocytic defects in neuroendocrine cells and neurons from TRPM7 knockout (KO) mice, indicating a role of TRPM7 in synaptic vesicle endocytosis. Our experiments further pinpoint the importance of TRPM7 as an ion channel in synaptic vesicle endocytosis. Ca2+ imaging detects a defect in presynaptic Ca2+ dynamics in TRPM7 KO neuron, suggesting an importance of Ca2+ influx via TRPM7 in synaptic vesicle endocytosis. Moreover, the short-term depression is enhanced in both excitatory and inhibitory synaptic transmission from TRPM7 KO mice. Taken together, our data suggests that Ca2+ influx via TRPM7 may be critical for short-term plasticity of synaptic strength by regulating synaptic vesicle endocytosis in neurons.

Funding

Functional characterization of Optimus prime

Directorate for Biological Sciences

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Role of vesicular TRPM7 channels in synaptic vesicle endocytosis

National Institute of Neurological Disorders and Stroke

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History

Citation

Jiang, Z. J., Li, W., Yao, L. H., Saed, B., Rao, Y., Grewe, B. S., McGinley, A., Varga, K., Alford, S., Hu, Y. S.Gong, L. W. (2021). TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis. eLife, 10, e66709-. https://doi.org/10.7554/eLife.66709

Publisher

eLife Sciences Publications, Ltd

Language

en

issn

2050-084X