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Tea Catechin Epigallocatechin gallate inhibits Streptococcus mutans Biofilm Formation by Suppressing gtf Genes

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journal contribution
posted on 27.06.2012, 00:00 authored by Xin Xu, Xue D. Zhou, Christine D. Wu
Objective: The anti-cariogenic properties of tea have been suggested for decades. Tea polyphenols, especially Epigallocatechin gallate (EGCG), have been shown to inhibit dental plaque accumulation, but the exact mechanisms are not clear at present. We hypothesize that EGCG suppresses gtf genes in S. mutans at the transcriptional level disrupting the initial attachment of S. mutans and thus the formation of mature biofilms. Design: In this study, the effect of EGCG on the sucrose-dependent initial attachment of S. mutans UA159 in a chemically defined medium was monitored over 4 h using a chamber slide model. The effects of EGCG on the aggregation and gtf B, C, D gene expression of S. mutans UA159 were also examined. Results: It was found that EGCG (7.8-31.25 μg/ml) exhibited dose-dependent inhibition of the initial attachment of S. mutans UA159. EGCG did not induce cellular aggregation of S. mutans UA159 at concentrations less than 78.125 μg/ml. Analysis of data obtained from real-time PCR showed that EGCG at sub-MIC level (15.6 μg/ml) significantly suppressed the gtf B, C, D genes of S. mutans UA159 compared with the non-treated control (p < 0.05). Conclusions: These findings suggest that EGCG may represent a novel, natural anti-plaque agent that inhibits the specific genes associated with bacterial biofilm formation without necessarily affecting the growth of oral bacteria.

Funding

This study was supported by the Department of Pediatric Dentistry, UIC College of Dentistry, Chicago, IL. Dr. Xin Xu is the recipient of a scholarship granted by the State Scholarship Fund, the China Scholarship Council.

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Publisher Statement

NOTICE: this is the author’s version of a work that was accepted for publication in Archives of Oral Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Archives of Oral Biology, Vol #, Issue #, (December 2011). http://dx.doi.org/10.1016/j.archoralbio.2011.10.021

Publisher

Elsevier

Language

en_US

issn

0003-9969

Issue date

01/12/2011

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