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The Mating-specific Gα Interacts with a Kinesin-14 and Regulates Pheromone-induced Nuclear Migration in Budding Yeast

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posted on 18.05.2022, 19:13 authored by Sofia V Zaichick, Metodi V Metodiev, Scott A Nelson, Oleksii Durbrovskyi, Edward Draper, John A Cooper, David StoneDavid Stone
As a budding yeast cell elongates toward its mating partner, cytoplasmic microtubules connect the nucleus to the cell cortex at the growth tip. The Kar3 kinesin-like motor protein is then thought to stimulate plus-end depolymerization of these microtubules, thus drawing the nucleus closer to the site where cell fusion and karyogamy will occur. Here, we show that pheromone stimulates a microtubule-independent interaction between Kar3 and the mating-specific Galpha protein Gpa1 and that Gpa1 affects both microtubule orientation and cortical contact. The membrane localization of Gpa1 was found to polarize early in the mating response, at about the same time that the microtubules begin to attach to the incipient growth site. In the absence of Gpa1, microtubules lose contact with the cortex upon shrinking and Kar3 is improperly localized, suggesting that Gpa1 is a cortical anchor for Kar3. We infer that Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating.

Funding

G Protein Regulation of A Microtubule Motor Protein in Yeast | Funder: National Science Foundation | Grant ID: MCB-0453964

History

Citation

Zaichick, S. V., Metodiev, M. V., Nelson, S. A., Durbrovskyi, O., Draper, E., Cooper, J. A.Stone, D. E. (2009). The Mating-specific Gα Interacts with a Kinesin-14 and Regulates Pheromone-induced Nuclear Migration in Budding Yeast. Molecular Biology of the Cell, 20(12), 2820-2830. https://doi.org/10.1091/mbc.e09-01-0069

Publisher

American Society for Cell Biology (ASCB)

Language

en

issn

1059-1524