The Mechanism of Pre-transfer Editing in Yeast Mitochondrial Threonyl-tRNA Synthetase
journal contribution
posted on 2013-11-22, 00:00 authored by Jiqiang Ling, Kaitlyn M. Peterson, Ivana Simonović, Dieter Söll, Miljan SimonovićBackground: The mechanism of pre-transfer
editing by which aaRSs regulate translational
fidelity is not well understood.
Results: Yeast mitochondrial ThrRS, MST1,
hydrolyzes seryl adenylate at the aminoacylation
active site more rapidly than the cognate
threonyl adenylate.
Conclusion: MST1 discriminates against serine
and reduces mischarging of threonine tRNA by
employing pre-transfer editing.
Significance: The mechanism of misactivation
and pre-transfer editing of serine by ThrRS is
provided.