Perez and Alfonso 2020.pdf (2.68 MB)

The conserved ASCL1/MASH-1 ortholog HLH-3 specifies sex-specific ventral cord motor neuron fate in caenorhabditis elegans

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journal contribution
posted on 09.06.2021, 14:54 by LM Perez, Aixa Alfonso
Neural specification is regulated by one or many transcription factors that control expression of effector genes that mediate function and determine neuronal type. Here we identify a novel role for one conserved proneural factor, the bHLH protein HLH-3, implicated in the specification of sex-specific ventral cord motor neurons in C. elegans. Proneural genes act in early stages of neurogenesis in early progenitors, but here, we demonstrate a later role for hlh-3. First, we document that differentiation of the ventral cord type C motor neuron class (VC) within their neuron class, is dynamic in time and space. Expression of VC class-specific and subclass-specific identity genes is distinct through development and is dependent on the VC position along the A-P axis and their proximity to the vulva. Our characterization of the expression of VC class and VC subclass-specific differentiation markers in the absence of hlh-3 function reveals that VC fate specification, differentiation, and morphology requires hlh-3 function. Finally, we conclude that hlh-3 cell-autonomously specifies VC cell fate.

History

Citation

Perez, L. M.Alfonso, A. (2020). The conserved ASCL1/MASH-1 ortholog HLH-3 specifies sex-specific ventral cord motor neuron fate in caenorhabditis elegans. G3: Genes, Genomes, Genetics, 10(11), 4201-4213. https://doi.org/10.1534/g3.120.401458

Publisher

Oxford University Press (OUP)

Language

en

issn

2160-1836