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The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication

journal contribution
posted on 2022-11-29, 21:59 authored by Samah Matmati, Mélina Vaurs, José M Escandell, Laetitia Maestroni, Toru NakamuraToru Nakamura, Miguel G Ferreira, Vincent Géli, Stéphane Coulon
Mammalian CST (CTC1-STN1-TEN1) complex fulfills numerous functions including rescue of the stalled replication forks and termination of telomerase action. In fission yeast lacking the CTC1 ortholog, the Stn1-Ten1 complex restricts telomerase action via its sumoylation-mediated interaction with Tpz1TPP1. We identify a small ubiquitin-like modifier (SUMO)-interacting motif (SIM) in the carboxyl-terminal part of Stn1 and show that this domain is crucial for SUMO and Tpz1-SUMO interactions. Point mutations in the SIM (Stn1-226) lead to telomere elongation, impair Stn1-Ten1 recruitment to telomeres, and enhance telomerase binding, revealing that Stn1 SIM domain contributes to the inhibition of telomerase activity at chromosome ends. Our results suggest that Stn1-Ten1 promotes DNA synthesis at telomeres to limit single-strand DNA accumulation. We further demonstrate that Stn1 functions in the replication of telomeric and subtelomeric regions in a Taz1-independent manner. Genetic analysis reveals that misregulation of origin firing and/or telomerase inhibition circumvents the replication defects of the stn1-226 mutant. Together, our results show that the Stn1-Ten1 complex has a dual function at telomeres by limiting telomerase action and promoting chromosome end replication.

Funding

Roles of Checkpoint and DNA Repair Proteins in Fission Yeast Telomere Maintenance | Funder: National Institutes of Health (National Institute of General Medical Sciences) | Grant ID: R01GM078253

History

Citation

Matmati, S., Vaurs, M., Escandell, J. M., Maestroni, L., Nakamura, T. M., Ferreira, M. G., Géli, V.Coulon, S. (2018). The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication. Science Advances, 4(5), eaar2740-. https://doi.org/10.1126/sciadv.aar2740

Publisher

American Association for the Advancement of Science (AAAS)

Language

en

issn

2375-2548