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Thlaspi arvense binds Cu(II) as a bis-(L-histidinato) complex on root cell walls in an urban ecosystem

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posted on 04.04.2016, 00:00 authored by Alain Manceau, Alexandre Simionovici, Martine Lanson, Jonathan Perrin, Remi Tucoulou, Sylvain Bohic, Sirine C. Fakra, Matthew A. Marcus, Jean-Philippe Bedell, Kathryn L. Nagy
Root cell walls accumulate metal cations both during acquisition from the environment and removal from the protoplast to avoid toxicity, but molecular forms of the metals under field conditions remain elusive. We have identified how copper is bound to cell walls of intact roots of native Thlaspi arvense by combining synchrotron X-ray fluorescence and absorption techniques (XANES and EXAFS) at the nano-, micro-, and bulk scales. The plants grew naturally in sediment in a stormwater runoff basin at copper concentrations typical of urban ecosystems. About 90% of acquired copper is bound in vivo to cell walls as a unique five-coordinate Cu(II)–bis(L-histidinato) complex with one L-histidine behaving as a tridentate ligand (histamine-like chelate) and the other as a bidentate ligand (glycine-like chelate). Tridentate binding of Cu(II) would provide thermodynamic stability to protect cells against copper toxicity, and bidentate binding may enable kinetic lability along the cell wall through protein–protein docking with the non-bonded imidazole group of histidine residues. EXAFS spectra are provided as ESI to facilitate further identification of Cu–histidine and distinction of Cu–N from Cu–O bonds in biomolecules.

Funding

Grants from the Envirhonalpes Program from the Re´gion Rhoˆne-Alpes and the EC2CO program of the CNRS provided support. We thank the OTHU (Field Observatory for Urban Water Management) Federation (http://www.graie.org/othu/) and the Grand Lyon Communaute´ Urbaine for field access, Olivier Proux for assistance on beamline FAME (BM30B) at the ESRF, and Lysiane Brocard from the Bordeaux Imaging Center at INRA for the preparation of the ultramicrotomic sections. Two anonymous reviewers provided thoughtful comments that were used in revising the manuscript. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.

History

Publisher

Royal Society of Chemistry

Language

en_US

issn

1756-5901

Issue date

01/01/2013

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