Efficacy of a Novel Microelectronic Device Against an Endodontic Biofilm in a Tooth Model
thesisposted on 2019-12-01, 00:00 authored by Edward Song
Introduction: This study was a follow-up on a previous experiment which showed a statistically significant reduction in bacterial viability when using electrochemical disinfection on a single-species bacterial biofilm cultured in a 96-well plate. The purpose of this study was to investigate the effectiveness of electrochemical disinfection when using a tooth model cultured with a mixed-species bacterial biofilm. Methods: A total of 60 single-canal permanent teeth were cut to 15mm in length and cultured with a mixed-species biofilm containing Enterococcus faecalis, Fusobacterium nucleatum, and Porphyromonas gingivalis. They were then divided into five groups. Group 1 tooth samples were irrigated with phosphate-buffered saline (PBS), group 2 tooth samples were irrigated with PBS in combination with electrochemical disinfection (EC), group 3 tooth samples were irrigated with 1.5% NaOCl, group 4 tooth samples were irrigated with 1.5% NaOCl in combination with EC, and group 5 tooth samples were irrigated with 6% NaOCl. Results: There was a statistically significant 80.7% reduction in bacterial viability in group 2 compared to group 1. There were no statistically significant differences with respect to bacterial viability between groups 2 - 5. Conclusions: EC was found to be effective in reducing bacterial viability in a tooth model when used with PBS; however, no statistically significant bacterial viability reduction was identified when EC was combined with NaOCl compared with EC alone or NaOCL alone.
AdvisorXie, QianJohnson, BradfordMathew, Mathew
Degree GrantorUniversity of Illinois at Chicago
Degree nameMS, Master of Science
Submitted dateDecember 2019