Evaluation of Plasma Lipoprotein Profiles in a Mediterranean Diet Intervention

Nonpharmacological interventions that target modifiable risk factors such as obesity, diet, sedentary lifestyle, and downstream metabolic correlates (e.g., dyslipidemia) are increasingly being investigated to prevent Alzheimer's disease (AD). The plasma lipoprotein profile is age, APOE-genotype, sex, and modifiable risk dependent.The current study used a precision medicine approach to study a potential AD biomarker and clinical intervention through a model of risk. Plasma samples were leveraged from a Mediterranean diet randomized controlled trial in a sample of female obese older adults who are predominately African American. Plasma lipoprotein profiles were quantified from pre- and post-intervention plasma samples using size exclusion chromatography and cholesterol assays. Our results indicated notable eulipidemic shifts in the plasma lipoprotein profile. There was an effect of decreased weight and adiposity on eulipidemic shifts. APOE signature profiles and patterns of lipoprotein remodeling were evident. Future work will further establish the plasma lipoprotein profiles as a biomarker with further examination of lipid/protein content and associations with cognition.